H-2Kb antibody
Reactivity: Mouse
FACS, IP, IF
Host: Mouse
Monoclonal
AF6
unconjugated
(3 references)-
- Target See all H-2Kb products
- H-2Kb (MHC Class I H-2Kb)
- Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This H-2Kb antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
- Brand
- BD Pharmingen™
- Characteristics
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The AF6-88.5 antibody reacts with the H-2Kb MHC class I alloantigen. Reactivity with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v) has not been observed.
BD Pharmingen™ Purified Mouse Anti-Mouse H-2Kb - Purified - Clone AF6-88.5 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Mouse C57BL splenocytes
- Clone
- AF6
- Isotype
- IgG2a kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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: "Single H2Kb, H2Db and double H2KbDb knockout mice: peripheral CD8+ T cell repertoire and anti-lymphocytic choriomeningitis virus cytolytic responses." in: European journal of immunology, Vol. 29, Issue 4, pp. 1243-52, (1999) (PubMed).
: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, (1983) (PubMed).
: "Flow cytometry as an analytical and preparative tool in immunology." in: Journal of immunological methods, Vol. 50, Issue 3, pp. R85-112, (1982) (PubMed).
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: "Single H2Kb, H2Db and double H2KbDb knockout mice: peripheral CD8+ T cell repertoire and anti-lymphocytic choriomeningitis virus cytolytic responses." in: European journal of immunology, Vol. 29, Issue 4, pp. 1243-52, (1999) (PubMed).
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- Target
- H-2Kb (MHC Class I H-2Kb)
- Alternative Name
- H-2Kb (H-2Kb Products)
- Synonyms
- H-2K antibody, H-2K(d) antibody, H2-K antibody, K-f antibody, histocompatibility 2, K1, K region antibody, H2-K1 antibody
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