MHC Class I (H-2Kb/H-2Db) antibody (Biotin)
Reactivity: Mouse
FACS
Host: Mouse
Monoclonal
28-8
Biotin
(3 references)-
- Target
- MHC Class I (H-2Kb/H-2Db)
- Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
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The 28-8-6 antibody reacts with the H-2K[b] and H-2D[b] MHC class I alloantigens. The reaction with H-2K[b] has been reported to be stronger than that with H-2D[b]. Its recognition of H-2D[b] is dependent upon the presence of β2 microglobulin. Cross-reactivity with an epitope on the N-terminal domains, α1 and α2, of the H-2D[d] MHC class I alloantigen has also been described. Reactivity with other haplotypes (e.g., f, k, p, q, r, s) has not been observed.
BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]/H-2D[b] - Biotin - Clone 28-8-6 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- C3H.SW mouse splenocytes
- Clone
- 28-8
- Isotype
- IgG2a kappa
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-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).
: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).
: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).
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: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).
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- Target
- MHC Class I (H-2Kb/H-2Db)
- Alternative Name
- H-2K[b]/H-2D[b]
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