H-2Kb antibody (Biotin)

Catalog No. ABIN2689541

Product Details anti-H-2Kb Antibody (Biotin)

Target: H-2Kb (MHC Class I H-2Kb)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H-2Kb antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: BD Pharmingen™

Characteristics: The AF6-88.5 antibody reacts with the H-2Kb MHC class I alloantigen. Reactivity with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v) has not been observed. Immunohistochemistry of H-2Kb positive cells. Frozen sections of mouse thymus were reacted with the anti-H2Kb antibody. Cells expressing the MHC class I molecule H-2Kb can be identified by the intense brown labeling of their cell membranes. Magnification 20X.

BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b] - Biotin - Clone AF6-88.5 - Isotype Mouse IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse C57BL splenocytes

Clone: AF6

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-H-2Kb antibody (Biotin) (ABIN2689541)

Loken, Stall: "Flow cytometry as an analytical and preparative tool in immunology." in: Journal of immunological methods, Vol. 50, Issue 3, pp. R85-112, (1982) (PubMed).

Target Details for H-2Kb

Target: H-2Kb (MHC Class I H-2Kb)

Alternative Name: H-2K[b] (H-2Kb Products)

Synonyms: H-2K antibody, H-2K(d) antibody, H2-K antibody, K-f antibody, histocompatibility 2, K1, K region antibody, H2-K1 antibody