Cutaneous Lymphocyte Antigen antibody
Reactivity: Human, Mouse
FACS, IHC (f), IHC (fro)
Host: Rat
Monoclonal
HECA
unconjugated
(3 references)-
- Target
- Cutaneous Lymphocyte Antigen
- Reactivity
- Human, Mouse
- Host
- Rat
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- BD Pharmingen™
- Characteristics
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The HECA-452 monoclonal antibody specifically reacts with Cutaneous Lymphocyte Associated Antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as a ligand for selectins including CD62E (E-selectin, ELAM-1). CLA is expressed on high endothelium and on lymphocytes including most T lymphocytes infiltrating cutaneous sites of inflammation. Amongst peripheral blood cells, it is expressed on monocytes and granulocytes and a subset of lymphocytes. The HECA-452 antibody is also reportedly crossreactive with the mouse CLA carbohydrate epitope that is transiently expressed by PSGL-1/CD162 on activated T cells. A number of studies suggest that CLA plays an important role in supporting leucocyte adhesive interactions and migration into extravascular tissues during inflammation. Immunohistochemical staining of endothelial cells with CLA. Frozen section of human tonsil was stained with Purified Rat Anti-Human Cutaneous Lymphocyte Antigen (Cat. No. 550407) antibody. Endothelial cells of the high endothelial vessels can be identified by the intense brown labeling of their cell surface membranes. Amplification 40x.
BD Pharmingen™ Purified Rat Anti-Human Cutaneous Lymphocyte Antigen - Purified - Clone HECA-452 - Isotype Rat IgM, κ - Reactivity Hu, Ms - 1 mL - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Lymphocyte-depleted Stromal Preparation of Tonsil
- Clone
- HECA
- Isotype
- IgM kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).
: "The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140." in: Cell, Vol. 66, Issue 5, pp. 921-33, (1991) (PubMed).
: "ELAM-1 is an adhesion molecule for skin-homing T cells." in: Nature, Vol. 349, Issue 6312, pp. 796-9, (1991) (PubMed).
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: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).
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- Target
- Cutaneous Lymphocyte Antigen
- Background
- Synonyms: CLA, PSGL-1, CD162, P-selectin glycoprotein ligand 1, SELPL, SELPLG
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