Reacts with a 45 kD type I membrane molecule also known as APO-1, or Fas antigen. CD95 belongs to the tumor necrosis factor receptor (TNF-R) family of proteins and is expressed on various cells, including activated T cells and B cells. The interaction of CD95, Fas antigen, with Fas ligand is one of the many mechanisms that lead to apoptosis or programmed cell death. The interaction of CD95 expressing target cells with Fas ligand on cytotoxic T cells induces an apoptotic signal leading to target cell death. It has been reported that apoptosis is the mechanism used by the immune system in clonal deletion of self-reactive T cells during embryological development. Jurkat cells treated for 6 hours with CD95 (EOS9.1) Jurkat cells treated for 6 hours with mouse IgM isotype control
FAS
Reactivity: Human
WB, ELISA, IP
Host: Mouse
Monoclonal
1B6
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing no preservative.
Preservative
Without preservative
Handling Advice
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Krammer, Dhein, Walczak, Behrmann, Mariani, Matiba, Fath, Daniel, Knipping, Westendorp: "The role of APO-1-mediated apoptosis in the immune system." in: Immunological reviews, Vol. 142, pp. 175-91, (1995) (PubMed).
Nagata, Golstein: "The Fas death factor." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1449-56, (1995) (PubMed).
Rouvier, Luciani, Golstein: "Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity." in: The Journal of experimental medicine, Vol. 177, Issue 1, pp. 195-200, (1993) (PubMed).