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CD8b.2 antibody

CD8BP Reactivity: Mouse FACS, IF, IP, IHC (fro), IHC (p) Host: Rat Monoclonal 53 unconjugated
Catalog No. ABIN2689441
  • Target
    CD8b.2 (CD8BP) (CD8b Molecule Pseudogene (CD8BP))
    Reactivity
    Mouse
    Host
    Rat
    Clonality
    Monoclonal
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    BD Pharmingen™
    Characteristics
    The 53-5.8 antibody reacts with the β chain of the CD8 differentiation antigen (Ly-3.2 or Lyt-3.2) of most mouse strains, having weak reactivity with Ly-3.1 strains (e.g., AKR, C58, MRL, PL). The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56lck). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

    BD Pharmingen™ Purified Rat Anti-Mouse CD8b.2 - Purified - Clone 53-5.8 - Isotype Rat IgG1, κ - Reactivity Ms - 0.5 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse thymus or spleen
    Clone
    53
    Isotype
    IgG1 kappa
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Messaoudi, Guevara Patiño, Dyall, LeMaoult, Nikolich-Zugich: "Direct link between mhc polymorphism, T cell avidity, and diversity in immune defense." in: Science (New York, N.Y.), Vol. 298, Issue 5599, pp. 1797-800, (2002) (PubMed).

    Fujiura, Kawaguchi, Kondo, Obana, Yamamoto, Nanno, Ishikawa: "Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, (1996) (PubMed).

    Sydora, Brossay, Hagenbaugh, Kronenberg, Cheroutre: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, (1996) (PubMed).

    Süss, Shortman: "A subclass of dendritic cells kills CD4 T cells via Fas/Fas-ligand-induced apoptosis." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1789-96, (1996) (PubMed).

    Wu, Vremec, Ardavin, Winkel, Süss, Georgiou, Maraskovsky, Cook, Shortman: "Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation." in: European journal of immunology, Vol. 25, Issue 2, pp. 418-25, (1995) (PubMed).

    Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, (1995) (PubMed).

    Nakayama, Nakayama, Negishi, Kuida, Louie, Kanagawa, Nakauchi, Loh: "Requirement for CD8 beta chain in positive selection of CD8-lineage T cells." in: Science (New York, N.Y.), Vol. 263, Issue 5150, pp. 1131-3, (1994) (PubMed).

    Wang, Klein: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, (1994) (PubMed).

    ORourke, Mescher: "The roles of CD8 in cytotoxic T lymphocyte function." in: Immunology today, Vol. 14, Issue 4, pp. 183-8, (1993) (PubMed).

    Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

    Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).

    Murosaki, Yoshikai, Ishida, Nakamura, Matsuzaki, Takimoto, Yuuki, Nomoto: "Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes." in: International immunology, Vol. 3, Issue 10, pp. 1005-13, (1992) (PubMed).

    Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).

    MacDonald, Schreyer, Howe, Bron: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, (1990) (PubMed).

    Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

    Walker, Murray, Hogarth, Kelso, McKenzie: "Comparison of thymic and peripheral T cell Ly-2/3 antigens." in: European journal of immunology, Vol. 14, Issue 10, pp. 906-10, (1984) (PubMed).

    Ledbetter, Seaman, Tsu, Herzenberg: "Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity." in: The Journal of experimental medicine, Vol. 153, Issue 6, pp. 1503-16, (1981) (PubMed).

    Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

  • Target
    CD8b.2 (CD8BP) (CD8b Molecule Pseudogene (CD8BP))
    Alternative Name
    CD8b.2
    Synonyms
    CD8B2 antibody, CD8b2 molecule antibody, CD8B2 antibody
    Background
    Synonyms: Ly-3.2
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