CD86 antibody (Biotin)
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- Target See all CD86 Antibodies
- CD86
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD86 antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
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2331 (FUN-1) recognizes a 75 kDa cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (anti-CD86, Cat. No. 555663) recognize the same molecule, they react with different epitopes. CD86 is the second ligand for CD28 and CTLA-4 and may play an important role in co-stimulation of T cells in primary immune response. 2331 (FUN-1) blocks costimulation activity of CD86 in functional studies. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of Daudi cells analyzed on a FACScan
BD Pharmingen™ Biotin Mouse Anti-Human CD86 - Biotin - Clone 2331 (FUN-1) - Isotype Mouse IgG1, κ - Reactivity Hu - 100 Tests - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Clone
- 2331
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD86 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 20 μL
- Restrictions
- For Research Use only
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- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).
: "Congenital plasminogen deficiency caused by a Ser572 to Pro mutation." in: Blood, Vol. 82, Issue 2, pp. 475-80, (1993) (PubMed).
: "A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells." in: The Journal of pathology, Vol. 169, Issue 3, pp. 309-15, (1993) (PubMed).
: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
: "
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The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).
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- Target
- CD86
- Alternative Name
- CD86 (CD86 Products)
- Synonyms
- B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
- Background
- Synonyms: B70/B7-2
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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