CD77 antibody

Catalog No. ABIN2689395

Product Details anti-CD77 Antibody

Target: CD77

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD77 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with CD77, a small neutral glycosphingolipid, 1 kDa, also referred to as globotriaosylceramide (Gb3). CD77 is expressed on a subset of germinal center B-lymphocytes and not on peripheral blood lymphocytes, bone marrow or spleen. It has been described as a receptor for Shiga Toxin, produced by Shigella dysenteriae, and Verotoxin 1 (VT1), produced by some strains of E. coli. CD19 has been reported as a ligand for CD77 and these interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of Ramos cell line analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human CD77 - Purified - Clone 5B5 - Isotype Mouse IgM, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 5B5

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD77 antibody (ABIN2689395)

Lagresle, Bella, Daniel, Krammer, Defrance: "Regulation of germinal center B cell differentiation. Role of the human APO-1/Fas (CD95) molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5746-56, (1995) (PubMed).

Mangeney, Rousselet, Taga, Tursz, Wiels: "The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis." in: Molecular immunology, Vol. 32, Issue 5, pp. 333-9, (1995) (PubMed).

Fyfe, Cebra-Thomas, Mustain, Davie, Alley, Nahm: "Subpopulations of B lymphocytes in germinal centers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 7, pp. 2187-94, (1987) (PubMed).

Target Details for CD77

Target: CD77

Abstract: CD77 Products