The 5F/B9 antibody reacts with CD73 or Ecto-5'-nucleotidase (5'-NT), a 68- kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart. The expression of CD73 on mouse leukocytes has been well characterized. In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes, NK cells, and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes. The 5F/B9 mAb does not inhibit the enzymatic activity of 5'-NT. Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18 (Cat. no. 554829). Two-color analysis of the expression of CD73 on rat spleen leukocytes. LEW splenocytes were stained with FITC- conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and either purified mouse IgG1, κ isotype control mAb MOPC-31C Cat. no. 557273, Left panel) or purified mAb 5F/B9 (Right panel), followed by biotinylated anti-mouse IgG1 mAb A85-1 (Cat. no. 553441), then Streptavidin-APC (Cat. no. 554067). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
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