GP5 antibody

Catalog No. ABIN2689254

Product Details anti-GP5 Antibody

Target: GP5 (Glycoprotein V (Platelet) (GP5))

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This GP5 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The 1C2 antibody reacts with CD42d (Platelet Glycoprotein V, GPV), a glycoprotein of 74 kDa in the mouse and 88 kDa in the rat. GPV noncovalently associates with GPIb (CD42b and c) and GPIX (CD42a) to form a receptor for von Willebrand factor on megakaryocytes and resting platelets. The binding sites for von Willebrand factor and thrombin have been localized to the GPIbachain (CD42b) of the GPIb-V-IX complex. GPV is cleaved by thrombin to release a 69- kDa soluble fragment, which contains the epitope recognized by mAb 1C2. The 1C2 antibody does not cross-react with human platelets nor with other hematopoietic cells of the mouse and rat. Anti-mouse CD41 mAb MWReg30 (Cat. No. 553848, for the FITC conjugate) may be used with mAb 1C2 to monitor mouse megakaryocyte maturation.

BD Pharmingen™ Purified Hamster Anti-Mouse and Rat CD42d - Purified - Clone 1C2 - Isotype Armenian Hamster IgG3, λ1 - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: (C57BL/6 x DBA/2)F1 mouse platelets

Clone: 1C2

Isotype: IgG3 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-GP5 antibody (ABIN2689254)

Sato, Kiyokawa, Takada, Itagaki, Saito, Sekino, Suzuki, Taguchi, Mimori, Lanza, Fujimoto: "Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d)." in: Hybridoma, Vol. 19, Issue 6, pp. 455-61, (2001) (PubMed).

Sato, Kiyokawa, Taguchi, Suzuki, Sekino, Ohmi, Itagaki, Sato, Lepage, Lanza, Fujimoto: "Functional conservation of platelet glycoprotein V promoter between mouse and human megakaryocytes." in: Experimental hematology, Vol. 28, Issue 7, pp. 802-14, (2000) (PubMed).

Ravanat, Morales, Azorsa, Moog, Schuhler, Grunert, Loew, Van Dorsselaer, Cazenave, Lanza: "Gene cloning of rat and mouse platelet glycoprotein V: identification of megakaryocyte-specific promoters and demonstration of functional thrombin cleavage." in: Blood, Vol. 89, Issue 9, pp. 3253-62, (1997) (PubMed).

Takada, Saito, Kaneko, Iizuka, Kokai, Fujimoto: "Novel monoclonal antibody reactive with thrombin-sensitive 74-kDa glycoproteins present on platelets and megakaryocytes both from mouse and rat." in: Hybridoma, Vol. 14, Issue 4, pp. 361-7, (1996) (PubMed).

Target Details for GP5

Target: GP5 (Glycoprotein V (Platelet) (GP5))

Alternative Name: CD42d (GP5 Products)

Synonyms: lib antibody, GPV antibody, PLGPV antibody, CD42d antibody, glycoprotein V platelet L homeolog antibody, glycoprotein V platelet antibody, glycoprotein 5 (platelet) antibody, glycoprotein V (platelet) antibody, gp5.L antibody, GP5 antibody, Gp5 antibody

Background: Synonyms: Platelet Glycoprotein V