CD4 antibody
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- Target See all CD4 Antibodies
- CD4
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Reactivity
- Rhesus Monkey, Cynomolgus, Baboon
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD4 antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
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The L200 antibody reacts with the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. L200 antibody also cross-reacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both rhesus and cynomolgus macaque monkeys. Cross-reactivity on both lymphocytes and monocytes (weak) of baboon is also observed. The distribution on lymphocytes is similar for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of anti-CD4 reactivity on peripheral blood lymphocytes of rhesus macaque (Macaca mulatta) analyzed by flow cytometry
BD Pharmingen™ Purified Mouse Anti-Human CD4 - Purified - Clone L200 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Clone
- L200
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD4 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys." in: Toxicology, Vol. 105, Issue 1, pp. 81-90, (1996) (PubMed).
: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, (1994) (PubMed).
: "Baboon T cell lymphomas expressing the B cell-associated surface proteins CD40 and Bgp95." in: Journal of clinical immunology, Vol. 12, Issue 3, pp. 225-36, (1992) (PubMed).
: "Phenotypic and functional differences in NK and LAK cells in the peripheral blood of sooty mangabeys and rhesus macaques." in: Cellular immunology, Vol. 124, Issue 1, pp. 107-18, (1989) (PubMed).
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Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys." in: Toxicology, Vol. 105, Issue 1, pp. 81-90, (1996) (PubMed).
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- Target
- CD4
- Alternative Name
- CD4 (CD4 Products)
- Synonyms
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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