TLR4 /MD2 Complex antibody

Catalog No. ABIN2689150

Product Details

Target: TLR4 /MD2 Complex

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The MTS510 antibody reacts with the molecular complex of Toll-Like Receptor 4 and MD-2 (TLR4-MD-2) which is expressed on LPS responsive macrophages. TLR4, a member of the Toll-Like Receptor Family, has been renamed CD284 and identified to be the transmembrane signal-transducing portion of the receptor for LPS. TLR4 associates on the cell surface with CD14 and MD-2, a 0.7 kDa molecule which is anchored to the membrane via its physical association with TLR4. The association of MD-2 with TLR4 is required for recognition of LPS and the anti-mitotic compound Taxol, which mimics the action of LPS on mouse cells. MTS510 mAb detects TLR4-MD-2 on the surface of thioglycollate-elicited macrophages from all mouse strains tested (ie, BALB/c, C57BL/6, C3H/HeJ, C3H/HeN, and DBA/1), including the C3H/HeJ strain which expresses an LPS-resistant mutant TLR4. Expression of TLR4-MD-2 is down-regulated on peritoneal macrophages after exposure to LPS, correlating with the occurrence of LPS tolerance. TLR4-MD-2 is not detected on splenocytes or thymocytes. Expression of TLR4-MD-2 on peritoneal macrophages. Thioglycollate-elicited peritoneal macrophages from BALB/c mice were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified mAb MTS510 (right panel), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142). Staining was detected by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) then Streptavidin-PE (Cat. No. 554061). The macrophages were identified by staining with FITC-conjugated anti-mouse CD11b (Integrin αM chain) mAb M1/70 (Cat. No. 557396/553310). The total viable leukocytes are displayed. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD284/MD-2 Complex - Purified - Clone MTS510 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Pro-B cell line Ba/F3 expressing transfected BALB/c mouse TLR4 and MD-2

Clone: MTS510

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Akashi, Saitoh, Wakabayashi, Kikuchi, Takamura, Nagai, Kusumoto, Fukase, Kusumoto, Adachi, Kosugi, Miyake: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).

Beutler, Poltorak: "The sole gateway to endotoxin response: how LPS was identified as Tlr4, and its role in innate immunity." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 29, Issue 4 Pt 2, pp. 474-8, (2001) (PubMed).

Akashi, Shimazu, Ogata, Nagai, Takeda, Kimoto, Miyake: "Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3471-5, (2000) (PubMed).

Nomura, Akashi, Sakao, Sato, Kawai, Matsumoto, Nakanishi, Kimoto, Miyake, Takeda, Akira: "Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3476-9, (2000) (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).

Target Details

Target: TLR4 /MD2 Complex

Alternative Name: CD284/MD-2 Complex

Background: Synonyms: TLR4/MD-2 Complex