The ATM-2 antibody reacts with rat CD252 (OX-40 Ligand, OX-40L), a member of the TNF Ligand superfamily. Although there is controversy regarding mouse OX-40L expression, OX-40L has been shown to be expressed on human dendritic cells and mouse activated B lymphocytes. Rat OX-40L interacts with OX-40 Antigen (CD134) found predominantly on activated T cells. The OX-40L-OX-40 interaction is reciprocally costimulatory in that both T cells and B cells are activated in cross-linking. Rat OX-40L mRNA has been detected in various HTLV-I-transformed T-cell lines: F344-S1, WKA-S1, and ACI-S1. In addition, total rat spleen cells activated with 5 ng/mL PMA and 0.5 μg/mL ionomycin for two days showed OX-40L expression, as determined by flow cytometric analysis, with the ATM-2 mAb. As observed in the mouse and human systems, rat OX-40L also costimulates IL-2 production and proliferation of anti- D3-activated rat T cells. The ATM-2 mAb blocks the costimulatory activity of OX-40L on rat T- cell proliferation.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Ohshima, Tanaka, Tozawa, Takahashi, Maliszewski, Delespesse: "Expression and function of OX40 ligand on human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 8, pp. 3838-48, (1997) (PubMed).
Calderhead, Buhlmann, van den Eertwegh, Claassen, Noelle, Fell: "Cloning of mouse Ox40: a T cell activation marker that may mediate T-B cell interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5261-71, (1993) (PubMed).
Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, (1988) (PubMed).
Target
TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))