CXCR4 antibody

Catalog No. ABIN2689046

Product Details anti-CXCR4 Antibody

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 2B11/CXCR4 monoclonal antibody specifically reacts with mouse CD184, which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as Fusin and LESTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. Mouse CXCR4 shows 91 % homology at amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes. Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The mouse CXCR4 gene has been mapped to chromosome 1. Detection of CXCR4 expression on BALB/c thymocytes by purified 2B11/CXCR4. BALB/c thymocytes were stained with 0.5 μg/test of Purified Rat anti-Mouse CD184 using 3-step staining protocol outlined below and APC Rat anti-Mouse CD4 (Cat. No. 553051). The level of nonspecific staining was assessed by using Purified Rat IgG2b, κ Isotype Control(Cat. No. 553986). The quadrant markers for the bivariate dot plots were set based on the isotype control. Flow cytometry was performed on a FACScan™ Flow Cytometer .

BD Pharmingen™ Purified Rat Anti-Mouse CD184 (CXCR4) - Purified - Clone 2B11/CXCR4 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: GST-NCXCR4 fusion protein

Clone: 2B11-CXCR4

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CXCR4 antibody (ABIN2689046)

De La Luz Sierra, Gasperini, McCormick, Zhu, Tosato: "Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells." in: Blood, Vol. 110, Issue 7, pp. 2276-85, (2007) (PubMed).

Schabath, Müller, Schubel, Kremmer, Lipp, Förster: "The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 996-1004, (2000) (PubMed).

Förster, Kremmer, Schubel, Breitfeld, Kleinschmidt, Nerl, Bernhardt, Lipp: "Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1522-31, (1998) (PubMed).

Heesen, Berman, Benson, Gerard, Dorf: "Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5455-60, (1997) (PubMed).

Bleul, Farzan, Choe, Parolin, Clark-Lewis, Sodroski, Springer: "The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry." in: Nature, Vol. 382, Issue 6594, pp. 829-33, (1997) (PubMed).

Bleul, Wu, Hoxie, Springer, Mackay: "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 5, pp. 1925-30, (1997) (PubMed).

Hesselgesser, Halks-Miller, DelVecchio, Peiper, Hoxie, Kolson, Taub, Horuk: "CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons." in: Current biology : CB, Vol. 7, Issue 2, pp. 112-21, (1997) (PubMed).

Oberlin, Amara, Bachelerie, Bessia, Virelizier, Arenzana-Seisdedos, Schwartz, Heard, Clark-Lewis, Legler, Loetscher, Baggiolini, Moser: "The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1." in: Nature, Vol. 382, Issue 6594, pp. 833-5, (1997) (PubMed).

Feng, Broder, Kennedy, Berger: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, (1996) (PubMed).

Loetscher, Geiser, OReilly, Zwahlen, Baggiolini, Moser: "Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes." in: The Journal of biological chemistry, Vol. 269, Issue 1, pp. 232-7, (1994) (PubMed).

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184 CXCR4 (CXCR4 Products)

Synonyms: CD184 antibody, D2S201E antibody, FB22 antibody, HM89 antibody, HSY3RR antibody, LAP3 antibody, LCR1 antibody, LESTR antibody, NPY3R antibody, NPYR antibody, NPYRL antibody, NPYY3R antibody, WHIM antibody, Cmkar4 antibody, PB-CKR antibody, PBSF/SDF-1 antibody, Sdf1r antibody, CXC-R4-B antibody, CXCR-4-B antibody, cd184 antibody, cxcr4 antibody, fb22 antibody, hm89 antibody, hsy3rr antibody, lap3 antibody, lcr1 antibody, lestr antibody, npy3r antibody, npyr antibody, npyrl antibody, npyy3r antibody, xcxcr4 antibody, CXC-R4 antibody, CXCR-4 antibody, d2s201e antibody, whim antibody, CXC-R4-A antibody, CXCR-4-A antibody, xCXCR4 antibody, CXCR4 antibody, cb403 antibody, zgc:109863 antibody, cb824 antibody, C-X-C motif chemokine receptor 4 antibody, chemokine (C-X-C motif) receptor 4 antibody, C-X-C motif chemokine receptor 4 S homeolog antibody, C-X-C chemokine receptor type 4 antibody, C-X-C motif chemokine receptor 4 L homeolog antibody, chemokine (C-X-C motif), receptor 4b antibody, chemokine (C-X-C motif) receptor 4a antibody, CXCR4 antibody, Cxcr4 antibody, cxcr4.S antibody, cxcr4 antibody, LOC100049444 antibody, cxcr4.L antibody, cxcr4b antibody, cxcr4a antibody

Background: Synonyms: CXCR4, C-X-C chemokine receptor type 4, Fusin, LESTR, PB-CKR, Sdf1r

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events