CD16 antibody

Catalog No. ABIN2689003

This anti-CD16 antibody is a Mouse Monoclonal antibody detecting CD16 in FACS, IHC (fro), IHC (p) and IHC (zinc). Suitable for Human, Rhesus Monkey, Cynomolgus and Baboon. This Primary Antibody has been cited in 4+ publications.

Quick Overview for CD16 antibody (ABIN2689003)

Target: CD16

Reactivity: Human, Rhesus Monkey, Cynomolgus, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: 3G8

Product Details anti-CD16 Antibody

Brand: BD Pharmingen™

Characteristics: The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both FcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation. Immunohistochemical staining of human CD16 positive cells. Frozen sections of normal human spleen was stained with Purified Mouse Anti-Human CD16 (Cat. No. 550383) antibody. Cells positive for CD16 can be identified by intense brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Mouse Anti-Human CD16 - Purified - Clone 3G8 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human polymorphonuclear leukocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD16 antibody (ABIN2689003)

Vossebeld, Homburg, Roos, Verhoeven: "The anti-Fc gamma RIII mAb 3G8 induces neutrophil activation via a cooperative actin of Fc gamma RIIIb and Fc gamma RIIa." in: The international journal of biochemistry & cell biology, Vol. 29, Issue 3, pp. 465-73, (1997) (PubMed).

Wirthmueller, Kurosaki, Murakami, Ravetch: "Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain." in: The Journal of experimental medicine, Vol. 175, Issue 5, pp. 1381-90, (1992) (PubMed).

Stroncek, Skubitz, Plachta, Shankar, Clay, Herman, Fleit, McCullough: "Alloimmune neonatal neutropenia due to an antibody to the neutrophil Fc-gamma receptor III with maternal deficiency of CD16 antigen." in: Blood, Vol. 77, Issue 7, pp. 1572-80, (1991) (PubMed).

Fleit, Wright, Unkeless: "Human neutrophil Fc gamma receptor distribution and structure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 10, pp. 3275-9, (1982) (PubMed).

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: Synonyms: FcRIII, Fc-gamma RIII, FCG3, FCGR3, FCGRIII, FcγRIII, IGFR3

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process