Reacts with platelet derived growth factor (PDGF) receptor α, CD140a, a 170 kD single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. They are structurally similar to M-CSF receptor and CD117 (c-kit). Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells. PDGF plays a role in wound healing and it also acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils. Functional PDGF is secreted as a dimer, disulfide linked, A and B chains (PDGFAA, PDGF-BB or PDGF-AB). Binding of divalent PDGF induces receptor dimerization with three possible forms: αα, αβ, ββ. The PDGF receptor α-subunit binds both PDGF A and B chains, whereas the receptor β-subunit binds only PDGF B chains. Both receptor subunits can stimulate mitogenic responses, only the β subunit can induce chemotaxis. αR1 is specific for αPDGFR and does not cross react with βPDGFR. It immunoprecipitates human, monkey, rabbit, pig, dog and cat αPDGFR. It does not recognize hamster, rat or mouse αPDGFR. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of MG-63 (osteosarcoma cell line) cells analyzed by flow cytometry
BD Pharmingen™ Purified Mouse Anti-Human CD140a - Purified - Clone αR1 (also known as Alpha-R1) - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Bazenet, Kazlauskas: "The PDGF receptor alpha subunit activates p21ras and triggers DNA synthesis without interacting with rasGAP." in: Oncogene, Vol. 9, Issue 2, pp. 517-25, (1994) (PubMed).