The I87-419 antibody reacts with the mouse C3a protein. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation. This antibody is routinely tested by ELISA Detection. Other applications were tested by BD Biosciences Pharmingen during antibody development only or reported in the literature. Representative Mouse C3a standard curve. The curve was generated by a sandwich ELISA using the purified I87-1162 antibody as capture antibody, doubling dilutions of purified mouse C3a protein as standard, and biotinylated I87-419 antibody as detection antibody. The standard curve is displayed as the concentration of purified mouse C3a in ng/mL versus the microwell absorbance.
BD Pharmingen™ Biotin Rat Anti-Mouse C3a - Biotin - Clone I87-419 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.