The purified I87-1162 antibody reacts with the mouse C3a protein. The I87-1162 antibody is specific for a neoepitope exposed in mouse C3a/C3adesArg and does not cross-react with mouse C3. This capacity makes antibody I87-1162 a preferential capture antibody for direct detection of mouse C3a from plasma or serum samples in an ELISA sandwich assay. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation. Representative Mouse C3a standard curve. The curve was generated by a sandwich ELISA using the purified I87-1162 antibody (Cat. No. 558250) as capture antibody, doubling dilutions of purified mouse C3a protein (Cat. No. 558618) as standard, and biotinylated I87-419 antibody (Cat. No. 558251) as detection antibody. The standard curve is displayed as the concentration of purified mouse C3a in ng/mL versus the microwell absorbance.
BD Pharmingen™ Purified Rat Anti-Mouse C3a - Purified - Clone I87-1162 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen
ELISA) Hugli T.E. Structure and function of the anaphylatoxins.