Antibody H32-406 reacts with B-lymphocyte stimulator protein (Blys), also known as BAFF (B cell activating factor) and is a member of the TNF ligand superfamily. Members of the TNF receptor and ligand superfamily play a critical role in regulating immune responses. Blys is a type II membrane protein expressed on cells of myeloid lineage including monocytes, dendritic cells, and activated T cells. Blys exists in both a membrane-bound and soluble form. In its soluble form, Blys is a potent B cell growth factor in costimulation assays, suggesting its biological role in monocyte-driven B cell activation. Blys has also been linked to B cell hyperplasia and autoimmunity. Human Blys sandwich ELISA curve: The curve was generated by a sandwich ELISA using the purified H32-406 as capture antibody, doubling dilutions of recombinant human Blys protein and biotin H32-544 (Cat. No. 558290) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214)were used to develop the ELISA.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Craxton, Magaletti, Ryan, Clark: "Macrophage- and dendritic cell--dependent regulation of human B-cell proliferation requires the TNF family ligand BAFF." in: Blood, Vol. 101, Issue 11, pp. 4464-71, (2003) (PubMed).
Mackay, Schneider, Rennert, Browning: "BAFF AND APRIL: a tutorial on B cell survival." in: Annual review of immunology, Vol. 21, pp. 231-64, (2003) (PubMed).
Khare, Sarosi, Xia, McCabe, Miner, Solovyev, Hawkins, Kelley, Chang, Van, Ross, Delaney, Wang, Lacey, Boyle, Hsu: "Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3370-5, (2000) (PubMed).
Moore, Belvedere, Orr, Pieri, LaFleur, Feng, Soppet, Charters, Gentz, Parmelee, Li, Galperina, Giri, Roschke, Nardelli, Carrell, Sosnovtseva, Greenfield, Ruben, Olsen, Fikes, Hilbert: "BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 260-3, (1999) (PubMed).
Target
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))