ARF1 antibody

Catalog No. ABIN2688655

Product Details anti-ARF1 Antibody

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Oryza sativa, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity (Details): No cross-reactivity with: Microsporidia sp.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 21 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, At2g47170)

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB), 1 : 1000 (IF).
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-ARF1 antibody (ABIN2688655)

Wang, Ito, Uehara, Naito, Takano: "UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells." in: Journal of plant research, (2015) (PubMed).

Pertl, Schulze, Obermeyer: "The pollen organelle membrane proteome reveals highly spatial-temporal dynamics during germination and tube growth of lily pollen." in: Journal of proteome research, Vol. 8, Issue 11, pp. 5142-52, (2009) (PubMed).

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: Arf1 (ARF1 Products)

Synonyms: wu:fb33e02 antibody, wu:fb78h01 antibody, arf2 antibody, arf-1 antibody, MGC52573 antibody, ADP-RIBOSYLATION FACTOR antibody, ADP-RIBOSYLATION FACTOR 1A antibody, ADP-ribosylation factor 1 antibody, ATARF antibody, ATARF1 antibody, ATARFA1A antibody, F28C11.12 antibody, ARF1 antibody, arf1 antibody, ADP ribosylation factor 1 antibody, ADP-ribosylation factor 1 antibody, ADP ribosylation factor 1 L homeolog antibody, ADP ribosylation factor 5 L homeolog antibody, ADP ribosylation factor 1 pseudogene antibody, adp-ribosylation factor 1 antibody, ARF1 antibody, Arf1 antibody, arf1 antibody, arf1.L antibody, arf5.L antibody, NCU08340 antibody, TRIADDRAFT_63238 antibody, PAAG_08805 antibody, LOC100280562 antibody, LOC100280918 antibody, LOC474591 antibody, CLUG_01013 antibody

Background: The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.

Molecular Weight: 21 kDa (Arabidopsis thaliana)

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process