Wild type Listeriolysin O protein from Listeria monocytogenes
Characteristics
Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of L. monocytogenes, has been identified as a candidate antigen for a serological assay.
Purification
Polyclonal immunoglobulins purified by protein A affinity chromatography.
Immunogen
Recombinant Listeriolysin O protein expressed in E. coli
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Buffer
Supplied in 100 mM sodium citrate, 50 mM Tris and 0.05 % v/v glycerol.
Storage
4 °C
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Kastner, Dussurget, Archambaud, Kernbauer, Soulat, Cossart, Decker: "LipA, a tyrosine and lipid phosphatase involved in the virulence of Listeria monocytogenes." in: Infection and immunity, Vol. 79, Issue 6, pp. 2489-98, (2011) (PubMed).
Sun, ORiordan: "Branched-chain fatty acids promote Listeria monocytogenes intracellular infection and virulence." in: Infection and immunity, Vol. 78, Issue 11, pp. 4667-73, (2010) (PubMed).
Bahey-El-Din, Casey, Griffin, Gahan: "Lactococcus lactis-expressing listeriolysin O (LLO) provides protection and specific CD8(+) T cells against Listeria monocytogenes in the murine infection model." in: Vaccine, Vol. 26, Issue 41, pp. 5304-14, (2008) (PubMed).
Port, Freitag: "Identification of novel Listeria monocytogenes secreted virulence factors following mutational activation of the central virulence regulator, PrfA." in: Infection and immunity, Vol. 75, Issue 12, pp. 5886-97, (2008) (PubMed).
Lety, Frehel, Raynaud, Dupuis, Charbit: "Exploring the role of the CTL epitope region of listeriolysin O in the pathogenesis of Listeria monocytogenes." in: Microbiology (Reading, England), Vol. 152, Issue Pt 5, pp. 1287-1296, (2006) (PubMed).