5-Hydroxymethylcytosine antibody

Catalog No. ABIN2668981

Product Details anti-5-Hydroxymethylcytosine Antibody

Target: 5-Hydroxymethylcytosine (5-hmC)

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification: None

Immunogen: This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

References for anti-5-Hydroxymethylcytosine antibody (ABIN2668981)

Zhong, Wang, Li, Zhang, An, Hou: "Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro." in: Scientific reports, Vol. 7, pp. 43539, (2017) (PubMed).

Page, Paoli, Moran Salvador, White, French, Mann: "Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape." in: Journal of hepatology, Vol. 64, Issue 3, pp. 661-73, (2016) (PubMed).

Pegoraro, Bafna, Davies, Shuker, Tauber: "DNA methylation changes induced by long and short photoperiods in Nasonia." in: Genome research, Vol. 26, Issue 2, pp. 203-10, (2016) (PubMed).

Marina, Sturgill, Bailly, Thenoz, Varma, Prigge, Nanan, Shukla, Haque, Oberdoerffer: "TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing." in: The EMBO journal, Vol. 35, Issue 3, pp. 335-55, (2016) (PubMed).

Brasa, Mueller, Jacquemont, Hahne, Rozenberg, Peters, He, McCormack, Gasparini, Chibout, Grenet, Moggs, Gomez-Mancilla, Terranova: "Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome." in: Clinical epigenetics, Vol. 8, pp. 15, (2016) (PubMed).

Shi, Yu, Luo, Zhang, Shi, Feng, Chen, He: "Loss of 5-Hydroxymethylcytosine Is an Independent Unfavorable Prognostic Factor for Esophageal Squamous Cell Carcinoma." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153100, (2016) (PubMed).

Nestor, Ottaviano, Reinhardt, Cruickshanks, Mjoseng, McPherson, Lentini, Thomson, Dunican, Pennings, Anderton, Benson, Meehan: "Rapid reprogramming of epigenetic and transcriptional profiles in mammalian culture systems." in: Genome biology, Vol. 16, pp. 11, (2015) (PubMed).

Vieweg, Dvorakova-Hortova, Dudkova, Waliszewski, Otte, Oels, Hajimohammad, Turley, Schorsch, Schuppe, Weidner, Steger, Paradowska-Dogan: "Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 31, (2015) (PubMed).

Her, Nelson-Holte, Maher: "Oxygen concentration controls epigenetic effects in models of familial paraganglioma." in: PLoS ONE, Vol. 10, Issue 5, pp. e0127471, (2015) (PubMed).

Thomson, Fawkes, Ottaviano, Hunter, Shukla, Mjoseng, Clark, Coutts, Murphy, Meehan: "DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) as a rapid method to generate genome wide epigenetic signatures." in: Scientific reports, Vol. 5, pp. 9778, (2015) (PubMed).

Gustafson, Yang, Dickson, Shao, Van Booven, Harbour, Liu, Wang: "Epigenetic reprogramming of melanoma cells by vitamin C treatment." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 51, (2015) (PubMed).

Wang, Sun, Jiao, Chong, Chen, Wang, Zhao, Zhou, Li: "Genome-wide 5-hydroxymethylcytosine modification pattern is a novel epigenetic feature of globozoospermia." in: Oncotarget, Vol. 6, Issue 9, pp. 6535-43, (2015) (PubMed).

Ma, Jiang, Deng, Gu, Wu, Li, Xia, Yang, Ye, Xiong, Guan: "D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth." in: Oncotarget, Vol. 6, Issue 11, pp. 8606-20, (2015) (PubMed).

Irie, Weinberger, Tang, Kobayashi, Viukov, Manor, Dietmann, Hanna, Surani: "SOX17 is a critical specifier of human primordial germ cell fate." in: Cell, Vol. 160, Issue 1-2, pp. 253-68, (2015) (PubMed).

Feng, Wang, Li, Zeng, Kuang, Li, Yue: "TET1-mediated different transcriptional regulation in prostate cancer." in: International journal of clinical and experimental medicine, Vol. 8, Issue 1, pp. 203-11, (2015) (PubMed).

Jin, Lu, Jelinek, Liang, Estecio, Barton, Issa: "TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells." in: Nucleic acids research, Vol. 42, Issue 11, pp. 6956-71, (2014) (PubMed).

Wu, Brenner: "Suppression of TET1-dependent DNA demethylation is essential for KRAS-mediated transformation." in: Cell reports, Vol. 9, Issue 5, pp. 1827-40, (2014) (PubMed).

Ahsan, Raabe, Haffner, Vaghasia, Warren, Quezado, Ballester, Nazarian, Eberhart, Rodriguez: "Increased 5-hydroxymethylcytosine and decreased 5-methylcytosine are indicators of global epigenetic dysregulation in diffuse intrinsic pontine glioma." in: Acta neuropathologica communications, Vol. 2, pp. 59, (2014) (PubMed).

Thomson, Lempiäinen, Hackett, Nestor, Müller, Bolognani, Oakeley, Schübeler, Terranova, Reinhardt, Moggs, Meehan: "Non-genotoxic carcinogen exposure induces defined changes in the 5-hydroxymethylome." in: Genome biology, Vol. 13, Issue 10, pp. R93, (2014) (PubMed).

Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine (5-hmC Products)

Target Type: Chemical