5-Hydroxymethylcytosine antibody

Catalog No. ABIN2668979

Product Details anti-5-Hydroxymethylcytosine Antibody

Target: 5-Hydroxymethylcytosine (5-hmC)

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purification: Protein G Chromatography

Immunogen: This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Clone: 59-1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-5-Hydroxymethylcytosine antibody (ABIN2668979)

Jia, Li, Cong, Yang, Sun, Parvizi, Zhao: "Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter." in: PLoS ONE, Vol. 8, Issue 5, pp. e63855, (2013) (PubMed).

Li, ONeill: "5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote." in: PLoS ONE, Vol. 8, Issue 5, pp. e63689, (2013) (PubMed).

Li, Zou, Jia, Zhao: "Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of mtDNA- and nuclear-encoded mitochondria genes in the liver of newborn piglets." in: BMC veterinary research, Vol. 9, pp. 87, (2013) (PubMed).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine (5-hmC Products)

Target Type: Chemical