Rpb1 CTD antibody (pSer7, Ser7)

Catalog No. ABIN2668542

Product Details anti-Rpb1 CTD Antibody

Target: Rpb1 CTD (RNA Polymerase II CTD)

Binding Specificity: pSer7, Ser7

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

Purification: Protein G Chromatography

Immunogen: This RNA pol II CTD pSer7 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 7.

Clone: 4E12

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Antibodies in solution can be stored at -80 °C for 2 years.

Expiry Date: 6 months

References for anti-Rpb1 CTD antibody (ABIN2668542)

Chung, Xu, Fuchs, Mund, Lange, Staege, Schubert, Bian, Dunkel, Eberharter, Regnard, Klinker, Meierhofer, Cozzuto, Winterpacht, Di Croce, Min, Will, Kinkley: "PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3." in: eLife, Vol. 5, (2016) (PubMed).

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Takahashi, Takigawa, Watanabe, Anwar, Shibata, Tomomori-Sato, Sato, Ranjan, Seidel, Tsukiyama, Mizushima, Hayashi, Ohkawa, Conaway, Conaway, Hatakeyama: "MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex." in: Nature communications, Vol. 6, pp. 5941, (2015) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD (Rpb1 CTD Products)

Molecular Weight: 240 kDa

Gene ID: 5430