Rpb1 CTD antibody (pSer5, Ser5)

Catalog No. ABIN2668541

Product Details anti-Rpb1 CTD Antibody

Target: Rpb1 CTD (RNA Polymerase II CTD)

Binding Specificity: pSer5, Ser5

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

Purification: None

Immunogen: This RNA pol II CTD pSer5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

Clone: 3E8

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Antibodies in solution can be stored at -80 °C for 2 years.

Expiry Date: 6 months

References for anti-Rpb1 CTD antibody (ABIN2668541)

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Ramos, Wijetunga, McLellan, Suzuki, Greally: "DNA demethylation by 5-aza-2'-deoxycytidine is imprinted, targeted to euchromatin, and has limited transcriptional consequences." in: Epigenetics & chromatin, Vol. 8, pp. 11, (2015) (PubMed).

Eifler, Shao, Bartholomeeusen, Fujinaga, Jäger, Johnson, Luo, Krogan, Peterlin: "Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 468-78, (2014) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD (Rpb1 CTD Products)

Molecular Weight: 240 kDa

Gene ID: 5430