Rpb1 CTD antibody (pSer2, Ser2)

Catalog No. ABIN2668539

Product Details anti-Rpb1 CTD Antibody

Target: Rpb1 CTD (RNA Polymerase II CTD)

Binding Specificity: pSer2, Ser2

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

Purification: Protein G Chromatography

Immunogen: This RNA pol II CTD pSer2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.

Clone: 3E10

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Antibodies in solution can be stored at -80 °C for 2 years.

Expiry Date: 6 months

References for anti-Rpb1 CTD antibody (ABIN2668539)

Chung, Xu, Fuchs, Mund, Lange, Staege, Schubert, Bian, Dunkel, Eberharter, Regnard, Klinker, Meierhofer, Cozzuto, Winterpacht, Di Croce, Min, Will, Kinkley: "PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3." in: eLife, Vol. 5, (2016) (PubMed).

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Catania, Pidoux, Allshire: "Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin." in: PLoS genetics, Vol. 11, Issue 3, pp. e1004986, (2015) (PubMed).

Eifler, Shao, Bartholomeeusen, Fujinaga, Jäger, Johnson, Luo, Krogan, Peterlin: "Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 468-78, (2014) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD (Rpb1 CTD Products)

Synonyms: 5 antibody, 8WG16 antibody, CG1554 antibody, CTD antibody, Dmel\\CG1554 antibody, H5 antibody, II antibody, L5 antibody, POL antibody, Pol II antibody, Pol II CTD antibody, Pol II Ser5P antibody, Pol II0[ser2] antibody, Pol II0[ser5] antibody, Pol II[ser2] antibody, Pol IIa antibody, Pol IIo antibody, Pol IIo[Ser2] antibody, Pol IIo[ser2] antibody, Pol IIo[ser5] antibody, Pol-IIa antibody, PolII antibody, PolIIo antibody, RNA Pol II antibody, RNA Pol II CTD antibody, RNA PolI 215 antibody, RNA PolII antibody, RNA pol II antibody, RNA pol IIo antibody, RNA polII antibody, RNAP antibody, RNAP II antibody, RNAP II LS antibody, RNAPII antibody, RNAPII0 antibody, RNApol2 antibody, RNApolII antibody, RPB1 antibody, RPII215 antibody, RpII antibody, Rpb1 antibody, Rpll215 antibody, Ubl antibody, dRPB1 antibody, dRpb1 antibody, l(1)10Ca antibody, l(1)DC912 antibody, l(1)DF912 antibody, l(1)G0040 antibody, l(1)L5 antibody, pol II antibody, polII antibody, RNA polymerase II 215kD subunit antibody, RpII215 antibody

Molecular Weight: 240 kDa

Gene ID: 5430