POLR2F antibody

Catalog No. ABIN2668523

Product Details anti-POLR2F Antibody

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human, Rat, Mouse, C. elegans, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Purification: Affinity Purified

Immunogen: This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.

Clone: 4H8

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: PBS containing 0.2 % gelatin, 0.01 % thimersol and 0.1 % sodium azide. Thimersol and sodium azide are highly toxic.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Antibodies in solution can be stored at -80 °C for 2 years.

Expiry Date: 6 months

References for anti-POLR2F antibody (ABIN2668523)

Li, Kannan, Trivett, Liao, Wu, Akagi, Symer: "An antisense promoter in mouse L1 retrotransposon open reading frame-1 initiates expression of diverse fusion transcripts and limits retrotransposition." in: Nucleic acids research, Vol. 42, Issue 7, pp. 4546-62, (2014) (PubMed).

Pang, Toh, He, Teissier, Ben Khalifa, Xue, Thierry: "A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c)." in: Oncogene, Vol. 33, Issue 31, pp. 4039-49, (2014) (PubMed).

Winston, Li, Sarna: "Chronic prenatal stress epigenetically modifies spinal cord BDNF expression to induce sex-specific visceral hypersensitivity in offspring." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 26, Issue 5, pp. 715-30, (2014) (PubMed).

Jha, Strahl: "An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair." in: Nature communications, Vol. 5, pp. 3965, (2014) (PubMed).

Plummer, Dan, Quinney, Hallmark, Phillips, Millar, Macpherson, Elcombe: "Identification of transcription factors and coactivators affected by dibutylphthalate interactions in fetal rat testes." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 132, Issue 2, pp. 443-57, (2013) (PubMed).

Akhrymuk, Kulemzin, Frolova: "Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II." in: Journal of virology, Vol. 86, Issue 13, pp. 7180-91, (2012) (PubMed).

Allison, Wamsley, Kumar, Li, Gray, Hart, Jones, Mayo: "Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-?B acetylation and transcription." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 42, pp. 16888-93, (2012) (PubMed).

Ellis, Shioda, Rosenthal, Coser, Shioda: "Masculine epigenetic sex marks of the CYP19A1/aromatase promoter in genetically male chicken embryonic gonads are resistant to estrogen-induced phenotypic sex conversion." in: Biology of reproduction, Vol. 87, Issue 1, pp. 23, 1-12, (2012) (PubMed).

Weishaupt, Attema: "A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip." in: Biological procedures online, Vol. 12, Issue 1, pp. 1-17, (2011) (PubMed).

Udelhoven, Leeser, Freude, Hettich, Laudes, Schnitker, Krone, Schubert: "Identification of a region in the human IRS2 promoter essential for stress induced transcription depending on SP1, NFI binding and ERK activation in HepG2 cells." in: Journal of molecular endocrinology, Vol. 44, Issue 2, pp. 99-113, (2010) (PubMed).

Udelhoven, Pasieka, Leeser, Krone, Schubert: "Neuronal insulin receptor substrate 2 (IRS2) expression is regulated by ZBP89 and SP1 binding to the IRS2 promoter." in: The Journal of endocrinology, Vol. 204, Issue 2, pp. 199-208, (2010) (PubMed).

Shirley, Rundhaug, Tian, Cullinan-Ammann, Lambertz, Conti, Fuchs-Young: "Transcriptional regulation of estrogen receptor-alpha by p53 in human breast cancer cells." in: Cancer research, Vol. 69, Issue 8, pp. 3405-14, (2009) (PubMed).

Song, Ro, Michaels, Park, McCarrey, Yan: "Many X-linked microRNAs escape meiotic sex chromosome inactivation." in: Nature genetics, Vol. 41, Issue 4, pp. 488-93, (2009) (PubMed).

Gatto, Rossi, Rossi, Kroening, Bonatti, Mallardo: "Epstein-Barr virus latent membrane protein 1 trans-activates miR-155 transcription through the NF-kappaB pathway." in: Nucleic acids research, Vol. 36, Issue 20, pp. 6608-19, (2008) (PubMed).

Raychaudhuri, Raychaudhuri, Thamotharan, Devaskar: "Histone code modifications repress glucose transporter 4 expression in the intrauterine growth-restricted offspring." in: The Journal of biological chemistry, Vol. 283, Issue 20, pp. 13611-26, (2008) (PubMed).

Leng, Butcher, Egan, Abi Abdallah, Denkers: "Toxoplasma gondii prevents chromatin remodeling initiated by TLR-triggered macrophage activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 489-97, (2008) (PubMed).

Coates, Lehnert, Sharma, Kindell, Gary: "Beryllium induces premature senescence in human fibroblasts." in: The Journal of pharmacology and experimental therapeutics, Vol. 322, Issue 1, pp. 70-9, (2007) (PubMed).

Romano, Delvoux, Fischer, Groothuis: "The PROGINS polymorphism of the human progesterone receptor diminishes the response to progesterone." in: Journal of molecular endocrinology, Vol. 38, Issue 1-2, pp. 331-50, (2007) (PubMed).

Kellerer, Schreiner, Stolt, Scholz, Bösl, Wegner: "Replacement of the Sox10 transcription factor by Sox8 reveals incomplete functional equivalence." in: Development (Cambridge, England), Vol. 133, Issue 15, pp. 2875-86, (2006) (PubMed).

Huffman, Geber: "Youth with disabilities and chronic illness: an international annotated bibliography." in: Pediatrician, Vol. 18, Issue 2, pp. 163-71, (1991) (PubMed).

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA Pol II (POLR2F Products)

Synonyms: HRBP14.4 antibody, POLRF antibody, RPABC2 antibody, RPB14.4 antibody, RPB6 antibody, 1810060D16Rik antibody, RNA polymerase II subunit F antibody, polymerase (RNA) II (DNA directed) polypeptide F antibody, POLR2F antibody, Polr2f antibody

Molecular Weight: 217 kDa

Gene ID: 5430

Pathways: Regulatory RNA Pathways