Nuclear Factor kappa B p50 (NFkB p50) antibody

Catalog No. ABIN2666083

Product Details

Target: Nuclear Factor kappa B p50 (NFkB p50)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Biochemical Assay (BCA)

Purification: The antibody was purified by affinity chromatography.

Clone: 4D1

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store between 2°C and 8°C.

Target Details

Target: Nuclear Factor kappa B p50 (NFkB p50)

Alternative Name: NF-B p50

Synonyms: NF-KB1 antibody, NF-kappaB antibody, NF-kappaB1 antibody, p105 antibody, p50 antibody, p50/p105 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, Nfkb1 antibody

Background: NF-κB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p50 is bound (with p65) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF- κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-κB p50 and has been shown to be useful for Western blotting.