Cutaneous Lymphocyte Antigen antibody (Pacific Blue)
Reactivity: Human, Mouse
FACS, IHC, IP
Host: Rat
Monoclonal
HECA-452
Pacific Blue
1 image
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- Target
- Cutaneous Lymphocyte Antigen
- Reactivity
- Human, Mouse
- Host
- Rat
- Clonality
- Monoclonal
- Conjugate
- Pacific Blue
- Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Purification
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
- Clone
- HECA-452
- Isotype
- IgM kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from prolonged exposure to light. Do not freeze.
- Storage
- 4 °C
- Storage Comment
- The antibody solution should be stored undiluted between 2°C and 8°C.
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- Target
- Cutaneous Lymphocyte Antigen
- Background
- Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.
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