MHC Class II I-Ek antibody (FITC)

Catalog No. ABIN2661929

Product Details anti-MHC Class II I-Ek Antibody (FITC)

Target: MHC Class II I-Ek

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II I-Ek antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Cross-Reactivity (Details): Cross-reacts with H-2d,p,r haplotypes. Mice of the H-2b,f,q,s haplotypes do not express I-E antigens. Cross-reacts with rat MHC class II antigen RT1D

Characteristics: The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Clone: 14-4-4S

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for MHC Class II I-Ek

Target: MHC Class II I-Ek

Alternative Name: I-Ek/rat RT1D (MHC Class II I-Ek Products)