Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)
Cross-Reactivity
Baboon, Cynomolgus, Rhesus Monkey
Purification
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.