Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD301b/MGL2 antibody (PE-Cy7)

MGL2 Reactivity: Mouse IHC, WB, CyTOF, ELISA (Capture) Host: Rat Monoclonal URA-1 PE-Cy7
Catalog No. ABIN2659223
  • Target See all CD301b/MGL2 (MGL2) products
    CD301b/MGL2 (MGL2) (Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2))
    Reactivity
    Mouse
    Host
    • 1
    Rat
    Clonality
    • 1
    Monoclonal
    Conjugate
    • 1
    This CD301b/MGL2 antibody is conjugated to PE-Cy7
    Application
    Immunohistochemistry (IHC), Western Blotting (WB), Cytometry by Time of Flight (CyTOF), ELISA (Capture)
    Purification
    The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
    Clone
    URA-1
    Isotype
    IgG2a lambda
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from prolonged exposure to light. Do not freeze.
    Storage
    4 °C
    Storage Comment
    The antibody solution should be stored undiluted between 2°C and 8°C.
  • Target
    CD301b/MGL2 (MGL2) (Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2))
    Alternative Name
    CD301b (MGL2 Products)
    Synonyms
    CD301b antibody, macrophage galactose N-acetyl-galactosamine specific lectin 2 antibody, Mgl2 antibody
    Background
    Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat. Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92 % for the intact sequence and 80 % for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2. So far, CD301a has been reported to be involved in recognition and endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived DCs.
You are here:
Support