TRA-1-60(R) antibody (Alexa Fluor 488)

Catalog No. ABIN2657591

Product Details

Target: TRA-1-60(R)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS), Cytometry by Time of Flight (CyTOF)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.

Clone: TRA-1-60-R

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details

Target: TRA-1-60(R)

Alternative Name: TRA-1-60-R

Background: TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.