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TRA-1-60(R) antibody (Alexa Fluor 488)

Reactivity: Human FACS, CyTOF Host: Mouse Monoclonal TRA-1-60-R Alexa Fluor 488
Catalog No. ABIN2657591
  • Target
    TRA-1-60(R)
    Reactivity
    Human
    Host
    • 1
    Mouse
    Clonality
    • 1
    Monoclonal
    Conjugate
    • 1
    Alexa Fluor 488
    Application
    Flow Cytometry (FACS), Cytometry by Time of Flight (CyTOF)
    Purification
    The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
    Clone
    TRA-1-60-R
    Isotype
    IgM kappa
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from prolonged exposure to light. Do not freeze.
    Storage
    4 °C
    Storage Comment
    The antibody solution should be stored undiluted between 2°C and 8°C
  • Target
    TRA-1-60(R)
    Alternative Name
    TRA-1-60-R
    Background
    TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.
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