IKZF3 antibody (Alexa Fluor 647)

Catalog No. ABIN2657113

Product Details anti-IKZF3 Antibody (Alexa Fluor 647)

Target: IKZF3 (IKAROS Family Zinc Finger 3 (Aiolos) (IKZF3))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKZF3 antibody is conjugated to Alexa Fluor 647

Application: Flow Cytometry (FACS)

No Cross-Reactivity: Mouse (Murine)

Purification: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Clone: 8B2

Isotype: IgG1 kappa, IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for IKZF3

Target: IKZF3 (IKAROS Family Zinc Finger 3 (Aiolos) (IKZF3))

Alternative Name: Aiolos (IKZF3 Products)

Synonyms: AIO antibody, AIOLOS antibody, ZNFN1A3 antibody, aio antibody, aiolos antibody, znfn1a3 antibody, IKZF3 antibody, 5830411O07Rik antibody, Aiolos antibody, Zfpn1a3 antibody, Znfn1a3 antibody, IKAROS family zinc finger 3 antibody, IKAROS family zinc finger 3 L homeolog antibody, IKZF3 antibody, ikzf3.L antibody, Ikzf3 antibody

Background: Aiolos, an Ikaros family member, is also known as Izkf3. It has a molecular weight of 58 kD and shows 70 % homology to the Ikaros isoform, Ik-1, particularly in the DNA binding and C-terminal domains. Aiolos can form a heteromer with Ikaros or exist as a homodimer. The homomeric complex is a potent transcriptional activator. Unlike Ikaros, Aiolos is expressed in late fetal thymus and adult lymphoid organs. Among the adult hematopoietic progenitors, Aiolos is expressed at low levels in mulitpotent but not in pluripotent stem cells. When these multipotent progenitors become more restricted to T and B lymphoid pathways there is an upregulation in Aiolos expression. The highest expression of Aiolos is detected in the spleen (especially in B-cells) followed by thymus and bone marrow, and cannot be detected in the non-lymphoid organs. Aiolos plays an important role in lymphocyte proliferation and differentiation.