TRA-2-49 antibody (Alexa Fluor 488)
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- Target
- TRA-2-49
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Alexa Fluor 488
- Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Activation (Act), Complement Mediated Cell Depletion (CMCD), Depletion (Dep)
- Purification
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Clone
- TRA-2-49-6E
- Isotype
- IgG1 kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from prolonged exposure to light. Do not freeze.
- Storage
- 4 °C
- Storage Comment
- The antibody solution should be stored undiluted between 2°C and 8°C
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- Target
- TRA-2-49
- Background
- TRA-2-49, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone, and kidney cells. It has been reported that the expression level of TRA-2-49 is decreased upon differentiation, therefore measurement of TRA-2-49 expression can be used to monitor the differentiation status of pluripotent stem cells.
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