UBE2V1 antibody (C-Term)

Catalog No. ABIN265158

Product Details anti-UBE2V1 Antibody

Target: UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2V1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detetcs UEV1A. UE1A is one of seven named isoforms of UEV1. UEV1A is located in the nucleus, while iosforms 3 and 5 are found in the membrane (7). This antibody may also recognize other isoforms of UEV1A.

Purification: Purified

Immunogen: Peptide corresponding to 15 amino acids near the C-terminus of human UEV1A

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 to 2 μg/mL. Postive Control: Jurkat Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for UBE2V1

Target: UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))

Alternative Name: UBE2V1 / CROC1 (UBE2V1 Products)

Background: Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).Synonyms: CROC-1, P/OKcl.19, TRAF6-regulated IKK activator 1 beta Uev1A, UBE2V, UEV-1, UEV1, Ubiquitin-conjugating enzyme E2 variant 1

Gene ID: 387522

NCBI Accession: NP_001027459

UniProt: Q13404

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades