VSX2 antibody (N-Term)

Catalog No. ABIN265011

Product Details anti-VSX2 Antibody

Target: VSX2 (Visual System Homeobox 2 (VSX2))

Binding Specificity: AA 1-131, N-Term

Reactivity: Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This VSX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes chx 10 (N-Term).

Purification: Purified

Immunogen: Antibody raised against a recombinant protein corresponding to amino acids 1 to 131 derived from the N terminal of the human Chx10 protein conjugated to GST.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5-1.0 mg/mL. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.08 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Can be shipped at ambient temperature.

References for anti-VSX2 antibody (ABIN265011)

Bai, Kiyama, Li, Wang: "Birth of cone bipolar cells, but not rod bipolar cells, is associated with existing RGCs." in: PLoS ONE, Vol. 9, Issue 1, pp. e83686, (2014) (PubMed).

Haynes, Luz-Madrigal, Reis, Echeverri Ruiz, Grajales-Esquivel, Tzekou, Tsonis, Lambris, Del Rio-Tsonis: "Complement anaphylatoxin C3a is a potent inducer of embryonic chick retina regeneration." in: Nature communications, Vol. 4, pp. 2312, (2013) (PubMed).

Tiwari, Hudson, Gattone, Miller, Chernoff, Belecky-Adams: "Meckelin 3 is necessary for photoreceptor outer segment development in rat Meckel syndrome." in: PLoS ONE, Vol. 8, Issue 3, pp. e59306, (2013) (PubMed).

Luo, Jin, Xie, Qiu, Li, Zou, Cai, Hozumi, Shima, Xiang: "Forkhead box N4 (Foxn4) activates Dll4-Notch signaling to suppress photoreceptor cell fates of early retinal progenitors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 9, pp. E553-62, (2012) (PubMed).

Sherry, Kanan, Hamilton, Hoffhines, Arbogast, Fliesler, Naash, Moore, Al-Ubaidi: "Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2." in: PLoS ONE, Vol. 7, Issue 6, pp. e39702, (2012) (PubMed).

Wakabayashi, Kosaka, Mori, Yamada: "Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 10, pp. 777-88, (2012) (PubMed).

Nittner, Lambertz, Clermont, Mestdagh, Köhler, Nielsen, Jochemsen, Speleman, Vandesompele, Dyer, Schramm, Schulte, Marine: "Synthetic lethality between Rb, p53 and Dicer or miR-17-92 in retinal progenitors suppresses retinoblastoma formation." in: Nature cell biology, Vol. 14, Issue 9, pp. 958-65, (2012) (PubMed).

Elshatory, Everhart, Deng, Xie, Barlow, Gan: "Islet-1 controls the differentiation of retinal bipolar and cholinergic amacrine cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 46, pp. 12707-20, (2007) (PubMed).

Osakada, Ooto, Akagi, Mandai, Akaike, Takahashi: "Wnt signaling promotes regeneration in the retina of adult mammals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 15, pp. 4210-9, (2007) (PubMed).

Yun, Sun, Wang, Rusovici, Castleberry, Hall, Shim: "LPA2 receptor mediates mitogenic signals in human colon cancer cells." in: American journal of physiology. Cell physiology, Vol. 289, Issue 1, pp. C2-11, (2005) (PubMed).

Dorsam, Graeler, Seroogy, Kong, Voice, Goetzl: "Transduction of multiple effects of sphingosine 1-phosphate (S1P) on T cell functions by the S1P1 G protein-coupled receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3500-7, (2003) (PubMed).

Target Details for VSX2

Target: VSX2 (Visual System Homeobox 2 (VSX2))

Alternative Name: VSX2 / CHX10 (VSX2 Products)

Synonyms: CHX10 antibody, HOX10 antibody, MCOP2 antibody, MCOPCB3 antibody, RET1 antibody, Chx10 antibody, Hox-10 antibody, Hox10 antibody, or antibody, VSX2 antibody, Vsx-2 antibody, alx antibody, chx10 antibody, c-ret antibody, cret antibody, etID315074.13 antibody, ret1 antibody, wu:fd13h01 antibody, visual system homeobox 2 antibody, ret proto-oncogene receptor tyrosine kinase antibody, Vsx2 transcription factor antibody, VSX2 antibody, Vsx2 antibody, vsx2 antibody, ret antibody

Background: Chx10 is a 46 kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.Synonyms: Ceh-10 homeodomain-containing homolog, HOX10, Homeobox protein CHX10, Visual system homeobox 2

Molecular Weight: 46 kDa

Gene ID: 12677

NCBI Accession: NP_031727

UniProt: Q61412

Pathways: Dopaminergic Neurogenesis