Farnesyl antibody

Catalog No. ABIN265009

Product Details

Target: Farnesyl

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Purification: Purified

Immunogen: Antibody developed using Farnesyl cysteine conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: Western Blot: Detects farnesyl motif derived from isoprenylated C-A-A-X sequence. Alsocross-reacts with geranylgeranyl motif. Detects KLH as well. Not suitable for Western blotdue to hydrophobicity of isoprenylated motif.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details

Target: Farnesyl

Target Type: Chemical

Background: Protein isoprenylation is a post-translational modification that affects about 0.5?% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A 1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.