M13 Bacteriophage gp3 antibody (Biotin)
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- Target
- M13 Bacteriophage gp3
- Reactivity
- Bacteriophage M13
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody recognizes M13 bacteriophage (gp3).
- Characteristics
- Synonyms: Attachment protein G3P, Gene 3 protein, Minor coat protein A
- Purification
- Protein A/G Chromatography
- Immunogen
- Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13phage coat proteins and mouse myeloma cells.
- Clone
- E1
- Isotype
- IgG2a
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- Application Notes
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Immunohistochemistry. Western blot: 1 - 5 μg/mL. Flow cytometry: 1 g/10^6 cells. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline with 0.08 % Sodium Azide as preservative
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
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The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- M13 Bacteriophage gp3
- Alternative Name
- M13 Bacteriophage G3P Minor Coat
- Target Type
- Phage Protein
- Background
- The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Attachment protein G3P, Gene 3 protein, Minor coat protein A
- Gene ID
- 927334
- UniProt
- P69168
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