Calpain antibody
Reactivity: Human, Cow
IP, WB, EIA
Host: Mouse
Monoclonal
28F3
unconjugated
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- Target See all Calpain products
- Calpain
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Reactivity
- Human, Cow
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Calpain antibody is un-conjugated
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Application
- Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)
- Characteristics
- Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1
- Purification
- Standard chromatographic techniques.
- Immunogen
- Calpain purified from human placenta.
- Clone
- 28F3
- Isotype
- IgG1
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anti-Calpain (AA 396-555), (Catalytic Subunit) antibody
Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- 0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as presevtative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. - Expiry Date
- 12 months
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- Target
- Calpain
- Abstract
- Calpain Products
- Synonyms
- CG18152 antibody, CG7563 antibody, Dmel\\CG7563 antibody, EST C antibody, calpain antibody, Tb08.28A12.350 antibody, Calpain-A antibody, hypothetical protein antibody, calpain 11 antibody, calpain antibody, CalpA antibody, T11A5.6 antibody, CAPN11 antibody, Tb927.8.8330 antibody
- Background
- The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2
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