Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Calpain antibody

Reactivity: Human, Cow WB, IP, EIA Host: Mouse Monoclonal 28F3 unconjugated
Catalog No. ABIN264387
  • Target See all Calpain products
    Calpain
    Reactivity
    • 4
    • 3
    • 1
    • 1
    Human, Cow
    Host
    • 4
    • 3
    Mouse
    Clonality
    • 4
    • 3
    Monoclonal
    Conjugate
    • 4
    • 1
    • 1
    • 1
    This Calpain antibody is un-conjugated
    Application
    • 6
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Characteristics
    Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1
    Purification
    Standard chromatographic techniques.
    Immunogen
    Calpain purified from human placenta.
    Clone
    28F3
    Isotype
    IgG1
  • Application Notes
    ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as presevtative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.
    Expiry Date
    12 months
  • Target
    Calpain
    Abstract
    Calpain Products
    Synonyms
    CG18152 antibody, CG7563 antibody, Dmel\\CG7563 antibody, EST C antibody, calpain antibody, Tb08.28A12.350 antibody, Calpain-A antibody, hypothetical protein antibody, calpain 11 antibody, calpain antibody, CalpA antibody, T11A5.6 antibody, CAPN11 antibody, Tb927.8.8330 antibody
    Background
    The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2
You are here:
Support