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HLA-DR antibody (FITC)

HLA-DR Reactivity: Human, Monkey FACS Host: Mouse Monoclonal 423L FITC

Catalog No. ABIN264299

Datasheet as PDF

Target See all HLA-DR products

HLA-DR
Reactivity
123

20

19

18

18

14

11

8

5

4

3

3

2
Human, Monkey
Host
103

13

10
Mouse
Compatible Secondaries
Clonality
119

6
Monoclonal
Conjugate
45

15

14

10

9

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HLA-DR antibody is conjugated to FITC
Application
110

34

25

20

15

13

12

10

6

6

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Characteristics

Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR

Clone

423L

Isotype

IgG2a
anti-HLA-DR (HLA-DR) antibody (FITC)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 FITC

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal TAL 1B5 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, WB, IP Host: Mouse Monoclonal MEM-12 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC Host: Mouse Monoclonal L243 unconjugated

anti-HLA-DR (HLA-DR) antibody (PE)
HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS Host: Mouse Monoclonal L243 PE

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, WB, IHC, IF, ISt, StM Host: Mouse Monoclonal SPM289 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, IP Host: Mouse Monoclonal HL-39 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func Host: Mouse Monoclonal L243 unconjugated

anti-HLA-DR (HLA-DR) antibody (PerCP)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PerCP

anti-HLA-DR (HLA-DR) antibody (APC)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 APC

Application Notes

Flow Cytometry. Monitoring of B cells in peripheral blood. Analysis of B subsets. Study of B cell activation. Study of B cell neoplasms.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol

PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Restrictions

For Research Use only
Buffer

PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date

12 months
Target

HLA-DR

Alternative Name

HLA Class II DR (HLA-DR Products)

Background

Identification of human B cell and T cell subsets associated with approximately 10 % of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR

Pathways

Human Leukocyte Antigen (HLA) in Adaptive Immune Response