TUBG1 antibody (AA 434-449)

Catalog No. ABIN263999

Product Details anti-TUBG1 Antibody

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Binding Specificity: AA 434-449

Reactivity: Plant, Protozoa, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: The antibody TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Characteristics: Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

Purification: Protein A Affinity Chromatography

Immunogen: Human gamma-tubulin peptide, amino acids 434-449AA Sequence: EYHAATRPDYISWGTQ

Clone: TU-32

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot. Immunocytochemistry. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 containing 15 mM Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TUBG1

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Alternative Name: TUBG1 / Tubulin gamma 1 (TUBG1 Products)

Background: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The γ-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing γ-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain

Gene ID: 7283

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase