Bovine Coronavirus antibody
BCoV
Reactivity: Bovine Coronavirus (BCoV)
EIA
Host: Mouse
Monoclonal
5A4
unconjugated
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- Target See all Bovine Coronavirus (BCoV) products
- Bovine Coronavirus (BCoV)
- Reactivity
- Bovine Coronavirus (BCoV)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Bovine Coronavirus antibody is un-conjugated
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Application
- Enzyme Immunoassay (EIA)
- Characteristics
- Synonyms: Spike glycoprotein, S glycoprotein
- Purification
- Protein G chromatography
- Purity
- > 95 % pure (SDS-PAGE)
- Immunogen
- Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/cmice.
- Clone
- 5A4
- Isotype
- IgG1
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- Application Notes
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ELISA. HIT (hemagglutination inhibition titer).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.8mg/mL (OD280nm E0.1%=1.4)
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Bovine Coronavirus (BCoV)
- Abstract
- BCoV Products
- Target Type
- Virus
- Background
- Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Synonyms: E2 protein, Peplomer protein, S glycoprotein, Spike glycoprotein
- UniProt
- P25193
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