SCP2 antibody (Internal Region)

Catalog No. ABIN263215

Product Details anti-SCP2 Antibody

Target: SCP2 (Sterol Carrier Protein 2 (SCP2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCP2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: Sterol carrier protein 2

Sequence: KNHKHSVNNP YSQ

Specificity: This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2, NP_001007099.1, NP_001180529.1, NP_001180528.1, NP_001180546.1 respectively),

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KNHKHSVNNPYSQ, from the internal region of the protein sequence according to NP_001007099.1, NP_002970.2, NP_001180528.1, NP_001180529.1, NP_001180546.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows speckled cytoplasm staining in hepatocytes. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SCP2

Target: SCP2 (Sterol Carrier Protein 2 (SCP2))

Alternative Name: SCP2 (SCP2 Products)

Background: SCP2 , sterol carrier protein 2, DKFZp686C12188, DKFZp686D11188, NLTP, NSL-TP, SCPX, nonspecific lipid-transfer protein, sterol carrier protein X

Gene ID: 6342, 20280, 25541

NCBI Accession: NP_001007099, NP_002970, NP_001180528, NP_001180529, NP_001180546

Pathways: C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process