CTDSPL antibody (Internal Region)

Catalog No. ABIN263205

Product Details anti-CTDSPL Antibody

Target: CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CTDSPL antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Purpose: CTDSPL

Sequence: QCNVSLKKQR SRS

Specificity: This antibody is expected to recognise both isoforms (NP_001008393.1, NP_005799.2).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1, NP_005799.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 28 kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2 μg/mL antibody staining (calculated Mwt of 29.9 kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and vesicles of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CTDSPL

Target: CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))

Alternative Name: CTDSPL (CTDSPL Products)

Background: CTDSPL, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like, C3orf8, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase-like protein, CTDSP-like, NIF-like protein, NLI-interacting factor 1, RB protein serine phosphatase fro

Gene ID: 10217

NCBI Accession: NP_001008393, NP_005799