CTDSPL antibody (Internal Region)

Catalog No. ABIN263205

Product Details anti-CTDSPL Antibody

Target: CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CTDSPL antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Purpose: CTDSPL

Sequence: QCNVSLKKQR SRS

Specificity: This antibody is expected to recognise both isoforms (NP_001008393.1, NP_005799.2).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1, NP_005799.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 28 kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2 μg/mL antibody staining (calculated Mwt of 29.9 kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and vesicles of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CTDSPL

Target: CTDSPL (CTD Small Phosphatase-Like Protein (CTDSPL))

Alternative Name: CTDSPL (CTDSPL Products)

Synonyms: C3orf8 antibody, HYA22 antibody, PSR1 antibody, RBSP3 antibody, SCP3 antibody, 2810418J22Rik antibody, AI426263 antibody, NFI1 antibody, NIF antibody, XNIF antibody, ctdspl antibody, ctdspl.L antibody, nif antibody, scp3 antibody, CTD small phosphatase like antibody, TIM23 translocase complex subunit Tim50 antibody, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like antibody, CTD small phosphatase like S homeolog antibody, CTDSPL antibody, SJAG_03560 antibody, Ctdspl antibody, ctdspl.S antibody

Background: CTDSPL, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like, C3orf8, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase-like protein, CTDSP-like, NIF-like protein, NLI-interacting factor 1, RB protein serine phosphatase fro

Gene ID: 10217

NCBI Accession: NP_001008393, NP_005799