TRPV5 antibody (Internal Region)
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- Target See all TRPV5 Antibodies
- TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This TRPV5 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Purpose
- TRPV5
- Sequence
- SHRGWEILRQ NT
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-SHRGWEILRQNT, from the internal region of the protein sequence according to NP_062815.2.
- Isotype
- IgG
- Top Product
- Discover our top product TRPV5 Primary Antibody
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- Application Notes
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Western Blot: Approx 85 kDa band observed in Human Pancreas lysates (calculated MW of 82.6 kDa according to NP_062815.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Kennedy
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF on Human: Kennedy BG et al. (2010) PMID: 20405023. This antibody has been successfully used in ICC on Mouse: Mariana Labarca et al. (2015) PMID: 2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium." in: Molecular vision, Vol. 16, pp. 665-75, (2010) (PubMed).
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Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium." in: Molecular vision, Vol. 16, pp. 665-75, (2010) (PubMed).
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- Target
- TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))
- Alternative Name
- TRPV5 (TRPV5 Products)
- Synonyms
- CaT2 antibody, Ecac1 antibody, CAT2 antibody, ECAC1 antibody, OTRPC3 antibody, D630033B11 antibody, TRPV5 antibody, ECAC antibody, cat2 antibody, xcat2 antibody, transient receptor potential cation channel, subfamily V, member 5 antibody, transient receptor potential cation channel subfamily V member 5 antibody, calcium transporter 2 L homeolog antibody, Trpv5 antibody, TRPV5 antibody, LOC100011049 antibody, trpv5 antibody, cat2.L antibody
- Background
- TRPV5 , transient receptor potential cation channel, subfamily V, member 5 , CAT2, ECAC1, OTRPC3, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3
- Gene ID
- 56302, 194352, 116469
- NCBI Accession
- NP_062815
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