DLG1 antibody (Internal Region)

Catalog No. ABIN263164

Product Details anti-DLG1 Antibody

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DLG1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: DLG1

Sequence: NSKTRDKGEI PDD

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-NSKTRDKGEIPDD

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 150 kDa in lysate of cell line Kelly after 0.3 μg/mL antibody staining (calculated MW of 103 kDa according to NP_004078.2). This molecular weight is observed by other sources. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and ER of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DLG1

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Alternative Name: DLG1 (DLG1 Products)

Synonyms: DLG1 antibody, Dlgh1 antibody, SAP97 antibody, B130052P05Rik antibody, E-dlg/SAP97 antibody, SAP-97 antibody, mKIAA4187 antibody, DLGH1 antibody, dJ1061C18.1.1 antibody, hdlg antibody, discs large MAGUK scaffold protein 1 antibody, discs, large homolog 1 (Drosophila) antibody, DLG1 antibody, Dlg1 antibody

Background: DLG1 , discs, large homolog 1 (Drosophila) , DKFZp761P0818 , DKFZp781B0426 , DLGH1 , SAP97 , dJ1061C18.1.1 , hdlg, discs large homolog 1 , discs, large homolog 1 , presynaptic protein SAP97 , synapse-associated protein 97

Gene ID: 1739

NCBI Accession: NP_001091894, NP_004078

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response