AIF antibody (C-Term)
-
- Target See all AIF (AIFM1) Antibodies
- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
-
Binding Specificity
- C-Term
-
Reactivity
- Human, Mouse, Rat, Pig
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This AIF antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
- Purpose
- AIFM1
- Sequence
- NEVAKLFNIH ED
- Specificity
- This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence
- Isotype
- IgG
- Top Product
- Discover our top product AIFM1 Primary Antibody
-
-
- Application Notes
-
Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.
Western Blot: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
-
Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
- Alternative Name
- AIFM1 (AIFM1 Products)
- Background
- AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
- Gene ID
- 9131, 26926, 83533
- NCBI Accession
- NP_004199, NP_665811, NP_665812, NP_001124318
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
-