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Vimentin antibody (C-Term)

Name: Vimentin antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Price: 469.28 USD
Availability: InStock

Verified VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

ABIN2613493 (0. (Vimentin antibody (C-Term))

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin antibody (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin antibody (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin antibody (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin antibody (C-Term))

FACS

5 images

Catalog No. ABIN2613493

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

18

16

16

15

14

11

9

7

7

4

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
341

132

129

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
195

130

11

6

6

5

1
Goat
Compatible Secondaries
Clonality
210

143
Polyclonal
Conjugate
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
285

129

105

99

95

64

46

40

39

33

23

19

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose

Vimentin

Sequence

QVINETSQHH DDLE

Cross-Reactivity

Cow, Dog, Human, Mouse, Pig, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Isotype

IgG

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VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

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VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

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VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

Application Notes

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

Comment

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Vimentin (VIM)

Alternative Name

VIM (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

VIM, vimentin, FLJ36605

Gene ID

7431, 22352, 81818

NCBI Accession

NP_003371

Pathways

Caspase Cascade in Apoptosis