Nucleostemin antibody (Internal Region)

Catalog No. ABIN2613433

Product Details anti-Nucleostemin Antibody

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Nucleostemin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: GNL3

Sequence: EKNNAQSIRA IKGPH

Specificity: This antibody is expected to recognize both reported isoforms (NP_055181.3, NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65+75 kDa bands observed in nuclear lysates of cell lines HeLa, HepG2 and K562 (calculated MW of 62.0 kDa according to NP_055181.3). The observed 75 kDa band likely represents the acetylated form. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Nucleostemin

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Alternative Name: GNL3 (GNL3 Products)

Synonyms: id:ibd2914 antibody, nstm antibody, wu:fb38a04 antibody, wu:fc55d07 antibody, zgc:123093 antibody, e2ig3 antibody, C77032 antibody, E2IG3 antibody, NNP47 antibody, NS antibody, BC037996 antibody, Ns antibody, guanine nucleotide binding protein-like 3 (nucleolar) antibody, G protein nucleolar 3 antibody, guanine nucleotide binding protein-like 3 (nucleolar) L homeolog antibody, gnl3 antibody, GNL3 antibody, Gnl3 antibody, gnl3.L antibody

Background: GNL3, guanine nucleotide binding protein-like 3 (nucleolar), C77032, E2IG3, NNP47, NS, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide-binding protein-like 3, novel nucleolar protein 47, nucleolar GTP-binding pr

Gene ID: 26354

NCBI Accession: NP_055181, NP_996561